Detection of homodimer formation of CD99 through extracelluar

mcd99Clones expressing mCD99 were harvested either by trypsin or using 10 mM EDTA in Hanks’ balanced salt solution (HBSS). Cells were washed twice in phosphate-bufered saWhen coexpressed, mCD99 formed heterodimers with mCD99L2, as well as homodimers, and the heterodimers were localized more efficiently at the plasma membrane